Vol. 2 No. 1 (2026): JPEPT
Articles

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BATANG BROTOWALI (Tinospora crispa L.) TERHADAP BAKTERI Staphylococcus epidemidis: ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF BROTOWALI STEM (Tinospora crispa L.) AGAINST Staphylococcus epidemicis BACTERIA

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  • Sulistiyani Rahmawati,
  • Andi Andi Zsazsa Rafiatul Mukhlis,
  • Raisha Hamiddani Syaiful,
  • Rahmayani Maghfirah
Sulistiyani Rahmawati
Program Studi D-III Farmasi, STIKES Darul Azhar Batulicin, Kalimantan Selatan
Andi Andi Zsazsa Rafiatul Mukhlis
Program Studi D-III Farmasi, STIKES Darul Azhar Batulicin, Kalimantan Selatan
Raisha Hamiddani Syaiful
Program Studi D-III Farmasi, STIKES Darul Azhar Batulicin, Kalimantan Selatan
Rahmayani Maghfirah
Puskesmas Batulicin, Dinas Kesehatan Kabupaten Tanah Bumbu, Kalimantan Selatan

Published 2026-01-31

Keywords

  • Antibacterial,
  • Disc diffusion,
  • Staphylococcus epidermidis,
  • Tinospora crispa,
  • Zone of inhibition

Copyright (c) 2026 Sulistiyani Rahmawati, Andi Andi Zsazsa Rafiatul Mukhlis, Raisha Hamiddani Syaiful, Rahmayani Maghfirah (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Skin disease is a fairly common and serious health problem in Indonesia. This problem can occur due to a lack of public awareness of health, resulting in the transmission of diseases, including skin health disorders, such as Staphylococcus epidermidis bacteria. This bacteria causes swelling like acne. One of the plants commonly used is the Brotowali plant. This study aims to determine the antibacterial effectiveness of the ethanol extract of Brotowali stems (Tinospora crisa L.) in inhibiting the growth of Staphyloccocus epidermidis. The method used is an experimental method with a disc diffusion test method. The bacteria used are Staphyloccocus epidermidis bacteria. The test is divided into 5 treatment groups, namely a negative control of sterile distilled water, a positive control of tetracycline, dose I, dose II and dose III. The results of the inhibition zone diameter are indicated by the appearance of a clear area on the disk and data analysis using the ANOVA test followed by the LSD test. The results of the antibacterial inhibition zone diameter in each group were, positive control 19.28 mm, dose I 5.52 mm, dose II 7.88 mm, and dose III 15.32 mm. The results of the Anova test data analysis obtained an antibacterial p-value of 0.015 (α <0.05) there was a significant difference, while the results of the LSD test in the test obtained positive control results with all doses I, II, and III significantly different in inhibiting the growth of the bacteria.

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References

  1. Ahmad, R., et al. (2023). Phytochemical screening and antibacterial evaluation of Tinospora crispa extracts against pathogenic bacteria. Journal of Ethnopharmacology, 302, 115-122.
  2. Azzahra, S., Ramadhan, F., & Siregar, I. (2023). Eksplorasi bahan alam sebagai alternatif antibiotik dalam mengatasi resistensi bakteri penyebab jerawat. Jurnal Farmasi dan Herbal, 5(1), 12-21.
  3. Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582. https://doi.org/10.1128/CMR.12.4.564
  4. Fathmah, E. N., Pujiyanto, S., & Raharjo, B. (2019). Aktivitas Antibakteri Ekstrak Etanol dan Etil Asetat Batang Tanaman Brotowali (Tinospora crispa, L. Miers) terhadap Bakteri Escherichia coli Enteropatogenik (EPEC) Penyebab Penyakit Diare. Berkala Bioteknologi, 2(1). Retrieved from https://ejournal2.undip.ac.id/index.php/bb/article/view/27583
  5. Fitriani, L., dkk. (2026). Uji Efektivitas Ekstrak Tanaman Herbal terhadap Bakteri Penyebab Akne Vulgaris. Jurnal Fitofarmaka Indonesia, 13(1), 12-20.
  6. Fitriani, L., Kusuma, S. A. F., & Siregar, I. (2026). Uji efektivitas ekstrak tanaman herbal terhadap bakteri penyebab akne vulgaris. Jurnal Fitofarmaka Indonesia, 13(1), 12-20. https://doi.org/10.33757/jfi.v13i1.482
  7. Nurwahdini Elvita., Mahdi Nur., Agustina Ani., FORMULATION AND EFFECTIVENESS TEST OF ANTISEPTIC LIQUID HAND WASHING SOAP PREPARATION FROM TARO LEAF ETHANOL EXTRACT (Colocasia esculenta). Journal Pharmacology Experimental and Pharmaceutical Technology. 1(1)
  8. Pelczar, M. J., & Chan, E. C. S. (1988). Dasar-dasar mikrobiologi (Hadioetomo, R. S., Imas, T., Tjitrosomo, S. S., & Angka, S. L., Penerjemah). UI Press.
  9. Pratama, M. (2005). Evaluasi aktivitas antibakteri bahan alam dan standardisasi zona hambat metode difusi. Penerbit Buku Kedokteran EGC.
  10. Putra, A. S., & Ramadhani, F. (2024). Aktivitas antibakteri ekstrak batang brotowali (Tinospora crispa L.) terhadap bakteri Gram positif dan Gram negatif: Sebuah tinjauan sistematis. Jurnal Farmasi Klinik dan Sains, 11(1), 45-53. https://doi.org/10.22146/jfks.v11i1.9214
  11. Radji, M. (2011). Buku ajar mikrobiologi: Panduan mahasiswa farmasi dan kedokteran. Penerbit Buku Kedokteran EGC.
  12. Rosyad, A., Handayani, S., & Utama, B. (2009). Identifikasi bakteri dan jamur penyebab jerawat (Acne vulgaris) pada remaja. Jurnal Dermatologi Indonesia, 17(4), 112-118.
  13. Rasyid, M., dkk. (2025). Pengaruh Jenis Pelarut pada Ekstraksi Bioaktif Tanaman Obat terhadap Daya Hambat Mikrobia. Jurnal Kimia dan Bahan Alam, 9(2), 88-95.
  14. Sari, D. P., & Wijaya, K. (2025). Mekanisme Kerusakan Dinding Sel Bakteri Gram Positif oleh Senyawa Polifenol Alam. Perhimpunan Mikrobiologi Indonesia, 21(3), 114-122.
  15. Sulistyo, B., & Hidayat, T. (2024). Faktor-Faktor yang Mempengaruhi Validitas Uji Mikrobiologi Metode Difusi Agar. Jurnal Laboratorium Medis, 7(4), 210-218.
  16. Utami, S., dkk. (2025). Pedoman Teknik Aseptik dan Sterilisasi Media Kultur di Laboratorium Mikrobiologi. Yogyakarta: Deepublish.
  17. Yulia, N., dkk. (2021). Aktivitas antibakteri kombinasi ekstrak etanol herba jeringau (Acorus calamus) dan batang brotowali (Tinospora crispa L.) terhadap pertumbuhan Streptococcus pyogenes. Jurnal Biomedika dan Kesehatan, 9(3), 145-152.